Embryonic stem cells are derived from the inner cell mass of blastocysts and can differentiate into any of over 200 cells in the body. Because of their origin and their ability to differentiate, embryonic stem cells provide valuable models for studying the effects of environmental chemicals on prenatal stages of development. As humans, we are more sensitive to environmental toxicants during prenatal development than at any other time in the life cycle. Our lab is currently using mouse and human embryonic stem cells to investigate the effects of environmental toxicants on early mammalian development.
We have recently used mouse embryonic stem cells (mESC) to study the effect of various types of cigarette smoke on cellular processes in stem cells. This study compared conventional and harm reduction products as well as mainstream and sidestream smoke. Mainstream smoke is inhaled by active smokers while sidestream smoke burns off the cigarette and is the major component of secondhand smoke or environmental tobacco smoke. Our first study with mESC showed that important biological processes such as cell-to-matrix attachment and proliferation are inhibited by smoke solutions from both conventional and harm reduction products. Moreover, MS and SS from harm reduction brands were usually more potent than smoke from the conventional brand. This unexpected result emphasizes the need to examine harm reduction products carefully and to fully access their safety before implementing their widespread use in tobacco control programs.
We are currently extending these experiments to human embryonic stem cells and developing assays to directly interrogate hESC with various types of tobacco products and other environmental chemicals.
More information on the Talbot Lab
HONORS AND AWARDS
Recipient of Chancellor's Award for Excellence in Mentoring Undergraduate Research, 1998-99
Elected Fellow, AAAS, 2001
Recipient of the Honor's Program Mentor of the Year Award, 2003
See publications on PubMed
S. Lin, V. Tran, and P. Talbot (2009) Comparison of toxicity of smoke from traditional and harm-reduction cigarettes using mouse embryonic stem cells as a novel model for preimplantation development. Hum. Reprod. 24: 386-397.
P. Talbot (2008) In Vitro Assessment of Reproductive Toxicity of Tobacco Smoke and Its Constituents Birth Defects Research (Part C) 84:61-72 (2008)
Boris Baibakov, Lyn Gauthier,P. Talbot, Tracy L. Rankin and Jurrien Dean. Sperm binding to the zona pellucida is not sufficient to induce acrosome exocytosis. Development 134, 933-943 (2007) doi:10.1242/dev.02752
P. Talbot, L. Fernandez. Scrambling the Egg Origin Dogma. Regenerative Medicine 2006; Volume 1, Number 5, September 2006, pp. 693-695(3)
K. Riveles, V. Tran, Ryan Roza, Derek Kwan, P. Talbot. Smoke from Traditional Commercial, Harm Reduction, and Research Brand Cigarettes Impairs Oviductal Functioning in Hamsters (Merocricetus auratus) In Vitro. Human Reproduction 2006; doi: 10.1093/humrep/del380
Richard Yu, M. Wu, S.Lin, Prue Talbot. Cigarette Smoke Toxicants Alter Growth and Survival of Cultured Mammalian Cells. Toxicological Sciences 2006; 93(1): 82-95.
Talbot P, Riveles K. Smoking and Reproduction: The Oviduct as a Target of Cigarette Smoke. Reproductive Biology and Endocrinology 2005; 3:25.
Liu M, Oh A, Calarco P, Yamada M, Coonrod SA, and Talbot P. Peptidylarginine Deiminase (PAD) is a Mouse Cortical Granule Protein that Plays a Role in Preimplantation Embryonic Development. Reproductive Biology and Endocrinology 2005; 3:24.
Gieseke C, and Talbot P. Cigarette Smoke Inhibits Hamster Oocyte Pickup by Increasing Adhesion between the Oocyte Cumulus Complex and Oviductal Cilia. Biology of Reproduction 2005; 73(3): 443-451.
Riveles K, Roza R, Talbot P. Phenols, Quinolines, Indoles, Benzene, and 2-Cyclopenten-1-ones are Oviductal Toxicants in Cigarette Smoke. Toxicological Sciences 2005; 86(1): 141-151.
Melkonian, G., Wang, J.L., Chung, J., Munoz, N., Talbot, P. CD44 and tenascin play critical roles in growth and vascular development of the chick chorioallantoic membrane and are targets of cigarette smoke. Anat Embryol (2004) 208:109–120.
Riveles, K., Roza R., Arey J., and Talbot, P.. 2004. Pyrazine derivatives in cigarette smoke inhibit hamster oviductal functioning. Reproductive Biology and Endocrinology 2:23.